What question did this study set out to answer?

The research aims to model sources of variability affecting transient expression in Nicotiana benthamiana experiments.

February 16, 2026Nature CommunicationsOpen Access

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression

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Authors

STSophia TangLawrence Berkeley National Laboratory MSMatthew J. SzarzanowiczLawrence Berkeley National Laboratory ALAmy LanctotLawrence Berkeley National Laboratory

Discussion

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Implication

Analysis highlights transient expression variability in Nicotiana benthamiana, suggesting optimal experimental design may improve results in synthetic biology.

Key Points

The research aims to model sources of variability affecting transient expression in Nicotiana benthamiana experiments.
Comprehensive analysis of variability in N. benthamiana agroinfiltration experiments.
Modeling sources of variability through data analysis of 1915 plants collected over three years.
Using power analysis to determine sample size requirements for effect size.
Identified the need for validating normalization methods in transient expression studies.
Found that distinct normalization schemes do not consistently reduce variability across experiments.
Provided guidelines for strategic experimental design to minimize variability.

Cite This Study

Tang et al. (2026) studied this question.

synapsesocial.com/papers/6992652ceb1f82dc367a0fd7 https://doi.org/10.1038/s41467-026-69458-1

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February 16, 2026Nature CommunicationsOpen Access

Causes and consequences of experimental variation in Nicotiana benthamiana transient expression

View Full Paper

Ask AI

Bookmark

Authors

STSophia TangLawrence Berkeley National Laboratory MSMatthew J. SzarzanowiczLawrence Berkeley National Laboratory ALAmy LanctotLawrence Berkeley National Laboratory

Discussion

Member takes

Implication

Analysis highlights transient expression variability in Nicotiana benthamiana, suggesting optimal experimental design may improve results in synthetic biology.

Key Points

The research aims to model sources of variability affecting transient expression in Nicotiana benthamiana experiments.
Comprehensive analysis of variability in N. benthamiana agroinfiltration experiments.
Modeling sources of variability through data analysis of 1915 plants collected over three years.
Using power analysis to determine sample size requirements for effect size.
Identified the need for validating normalization methods in transient expression studies.
Found that distinct normalization schemes do not consistently reduce variability across experiments.
Provided guidelines for strategic experimental design to minimize variability.

Cite This Study

Tang et al. (2026) studied this question.

synapsesocial.com/papers/6992652ceb1f82dc367a0fd7 https://doi.org/10.1038/s41467-026-69458-1

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