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November 14, 2025Plant Biotechnology JournalOpen Access

The Impacts of Environmental Factors and Culture Conditions on the Transient Expression of Recombinant Protein in Nicotiana benthamiana Under Controlled Environments

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Authors

RMRyo Matsuda

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Overview

This analysis demonstrates that environmental factors influence recombinant protein expression in Nicotiana benthamiana, suggesting optimal conditions are critical for biopharmaceutical production.

Key Points

  • The research examines how environmental factors and culture conditions affect recombinant protein expression.
  • Analyzed plant cultivation systems with controlled environments.
  • Investigated the relationship between leaf biomass and target protein accumulation.
  • Evaluated the impact of temperature and photoperiod on protein expression.
  • Higher plant density and extended photoperiod improved biomass productivity.
  • Temperature variations significantly affected target protein expression in leaves.
  • Treatment with ascorbic acid reduced necrosis severity associated with protein accumulation.

Cite This Study

Ryo Matsuda (2025) studied this question.

synapsesocial.com/papers/692519b4c0ce034ddc3544f8https://doi.org/10.1111/pbi.70453
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