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June 12, 2026Plant Cell ReportsOpen Access

Vector minimization and infiltration optimization for enhanced protein expression in Nicotiana benthamiana

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Authors

KPKulachatr PanyawechamontriHKHiroyuki KajiuraRMRyo Misaki

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Overview

Randomized trial shows enhanced protein expression in Nicotiana benthamiana, suggesting improved plant biotechnology methods.

Key Points

  • This research investigates how reducing vector size and optimizing infiltration conditions influences protein expression in Nicotiana benthamiana.
  • Reduced size of pBI121 vector by removing non-essential sequences to create modified constructs.
  • Evaluated effects of optimized infiltration buffer and co-expression with silencing suppressor p19, acetosyringone, and ascorbic acid.
  • Measured mRNA and protein levels of ꞵ-glucuronidase (GUS) and acid sphingomyelinase (ASM) proteins.
  • mpBI121 enhanced GUS and ASM mRNA expression by 2.02-fold and 2.05-fold, respectively, compared to pBI121.
  • Protein levels for GUS and ASM increased by 1.76-fold and 1.75-fold.
  • Optimized infiltration conditions led to the highest protein expression outputs.

Cite This Study

Panyawechamontri et al. (2026) studied this question.

synapsesocial.com/papers/6a2ba3fa8101cf8926f028b3https://doi.org/10.1007/s00299-026-03802-z
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