Spatial analysis demonstrates changes in gene expression and regulatory networks in transgenic mice during sex determination, highlighting epigenetic regulation.
Key Points
This research aims to understand the 3D chromatin landscape and its role in sex determination.
Analyzed fluorescence-activated cell-sorted embryonic mouse gonadal populations before and after sex determination.
Conducted Hi-C analyses to examine chromatin structures and topologically associating domains.
Utilized METALoci for spatial autocorrelation analysis of regulatory hubs.
Combined Hi-C data with ChIP-seq experiments to profile gene interactions.
Chromatin structures remained largely unchanged during sex determination, indicating preformed configurations.
Identified rewiring of chromatin interactions affecting hundreds of genes with sex-specific expression.
Discovered a 3D regulatory hub for Fgf9, where deletion led to male-to-female sex reversal in mutant mice.