What does this research mean for the field?

Calcium-silicate-based Bioceramic Sealer promotes a more favorable osteogenic response in MC3T3-E1 pre-osteoblasts compared to Zinc oxide eugenol sealer. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This study aims to explore the effects of endodontic sealers on osteogenic modulation and the underlying molecular mechanisms in MC3T3-E1 cells.

March 13, 2026Dentistry JournalOpen Access

In Vitro Assessment of Osteogenic Modulation and Molecular Responses Induced by Contemporary Endodontic Sealers in MC3T3-E1 Pre-Osteoblasts

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Authors

YMYuka MIYAMOTOAsahi University YKYuka KatoUniversity of California, Los Angeles RNRyan Brent NeedleUniversity of California, Los Angeles

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Implication

In Vitro Assessment Shows Osteogenic Modulation in Pre-Osteoblasts with Different Endodontic Sealers, Indicating Varied Biological Responses.

Key Points

This study aims to explore the effects of endodontic sealers on osteogenic modulation and the underlying molecular mechanisms in MC3T3-E1 cells.
Applied four commercial sealers to MC3T3-E1 pre-osteoblasts as standardized pellets.
Assessed calcium deposition through Alizarin Red S staining.
Evaluated MAPK activation using Western blotting.
Measured osteogenic-associated gene expression using qRT-PCR.
Analyzed the effects of MAPK inhibitors on treated cultures.
BC and Sealapex™ showed strong calcium deposits, while ZOE and AH26® showed minimal deposits.
BC and ZOE activated the MAPK pathway, with ZOE showing higher phosphorylation.
BC demonstrated greater cell viability and upregulated Runx2 and Osx, while ZOE impacted early cell attachment.
Inhibition of MAPK pathways in BC-treated cells reduced osteogenic gene expressions.

Cite This Study

MIYAMOTO et al. (2026) studied this question.

synapsesocial.com/papers/69b3acc502a1e69014cceb67 https://doi.org/10.3390/dj14030160

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Implication

In Vitro Assessment Shows Osteogenic Modulation in Pre-Osteoblasts with Different Endodontic Sealers, Indicating Varied Biological Responses.

In Vitro Assessment of Osteogenic Modulation and Molecular Responses Induced by Contemporary Endodontic Sealers in MC3T3-E1 Pre-Osteoblasts

Discussion

Member takes

Implication

Key Points

Cite This Study

In Vitro Assessment of Osteogenic Modulation and Molecular Responses Induced by Contemporary Endodontic Sealers in MC3T3-E1 Pre-Osteoblasts

Discussion

Member takes

Implication

Key Points

Cite This Study

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