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March 1, 1981Journal of Lipid ResearchOpen Access

Plasma lipoprotein changes resulting from immunologically blocked lipolysis

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Why the study?

Does immunologically blocked lipolysis alter plasma lipoprotein levels and composition in fasted roosters?

Population

Fasted roosters

Comparison

Intravenous injections of high titer goat… vs Control roosters

Design

Preclinical

Follow-up

8 hours

Authors

SBStephen R. BehrPennsylvania State UniversityJPJosef R. PatschPreventive CardiologyTFTrudy ForteChildren’s Institute

Discussion

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Overview

Should not alter clinical lipid management; hypothesis-generating for LPL-dependent lipoprotein remodeling in mammals.

Key Points

  • Investigate the role of lipoprotein lipase (LPL) in the generation and structural remodeling of plasma low density lipoprotein (LDL) and high density lipoprotein (HDL).
  • Administered intravenous injections of high-titer goat antiserum against purified chicken LPL into fasted roosters to quantitatively inhibit VLDL triglyceride catabolism.
  • Analyzed lipoprotein chemical composition, particle size via gel filtration and electron microscopy, and flotation characteristics via rate and equilibrium zonal ultracentrifugation across an 8-hour period.
  • Eight hours of LPL inhibition increased VLDL concentration over 10-fold, whereas LDL decreased 5-fold and HDL decreased by 48%, with cholesterol half-lives of 2.4 and 6 hours, respectively.
  • Experimental LDL contained 37% less phospholipid, 64% less cholesterol, and 2.3-fold more triglyceride; experimental HDL was smaller (Stokes' radius 44.9 Å vs. 55.4 Å) and denser (1.118 vs. 1.113 g/ml).
  • Rate zonal ultracentrifugation failed to detect any circulating LDL in experimental samples after 8 hours of LPL blockade.

Structured PICO

Does immunologically blocked lipolysis alter plasma lipoprotein levels and composition in fasted roosters?

P
Population
Fasted roosters
I
Intervention
Intravenous injections of high titer goat antiserum against highly purified chicken lipoprotein lipase (LPL)
C
Comparator
Control roosters
O
Outcome
Plasma lipoprotein changes (VLDL, LDL, HDL concentrations and composition)surrogate

In vivo inhibition of lipoprotein lipase in roosters demonstrates that LDL is a direct metabolic product of VLDL and LPL is crucial for transferring surface constituents from VLDL to HDL.

Cite This Study

Behr et al. (1981) studied this question.

synapsesocial.com/papers/69ccf700da2a6987e0cbfbd0https://doi.org/10.1016/s0022-2275(20)34958-0
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Formation of high density lipoprotein2-like particles during lipolysis of very low density lipoproteins in vitro.1978 · 508 citations
  2. 2PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT1951 · 318,678 citations
  3. 3Secretion of Cholesterol-Rich Lipoproteins by Perfused Livers of Hypercholesterolemic Rats1979 · 108 citations
  4. 4Isolation, chemical characterization, and biophysical properties of three different abnormal lipoproteins: LP-X1, LP-X2, and LP-X3.1977 · 77 citations
  5. 5Synthesis and release of low density lipoproteins by the isolated perfused pig liver.1977 · 45 citations