Focused ultrasound modifies extracellular vesicle composition in glioblastoma, suggesting new biomarkers for liquid biopsy.
Key Points
This study investigates how focused ultrasound-mediated blood brain barrier opening affects the release and composition of extracellular vesicles in glioblastoma.
Established orthotopic glioblastomas via intracranial implantation in a rodent model.
Utilized contrast-enhanced MRI for tumor screening and randomized into FUS treatment or sham groups.
Performed blood brain barrier opening using a neuronavigation-guided focused ultrasound system.
Collected plasma samples for extracellular vesicle isolation and proteomic analysis post-treatment.
No significant change in total plasma extracellular vesicle concentrations post-treatment.
Identified over 5,000 proteins, with 80% being unique to either tumor- or brain-derived extracellular vesicles.
Found 222 unique proteins in brain-derived and 290 in tumor-derived circulating extracellular vesicles post-FUS.
Pathway analysis indicated involvement in vesicle trafficking and immune regulatory pathways.