What question did this study set out to answer?

The aim is to develop a highly efficient transient expression system to produce high titer retroviral vectors.

January 1, 1995Nucleic Acids ResearchOpen Access

A transient three-plasmid expression system for the production of high titer retroviral vectors

View Full Paper

Ask AI

Bookmark

The aim is to develop a highly efficient transient expression system to produce high titer retroviral vectors.
Constructed MLV-based retroviral vectors with CMV promoter and SV40 origins of replication.
Used a highly transfectable human cell line with sodium butyrate to increase expression.
Separated gag-pol and env packaging components on distinct plasmids to minimize helper virus formation.
Achieved helper-free viral stocks of approximately 10^7 infectious units/ml.
Increased retroviral gene expression observed in SV40 T antigen carrying cell lines by 48 hours after transfection.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Randomized trial demonstrates enhanced gene expression in human cells using a three-plasmid system, indicating potential for increased retroviral vector production.

Cite This Study

Soneoka et al. (1995) studied this question.

synapsesocial.com/papers/69d8474752654bb436d18faf https://doi.org/10.1093/nar/23.4.628

View Full Paper

Ask AI

Bookmark

Implication

Randomized trial demonstrates enhanced gene expression in human cells using a three-plasmid system, indicating potential for increased retroviral vector production.

A transient three-plasmid expression system for the production of high titer retroviral vectors

Key Points

Discussion

Implication

Cite This Study

A transient three-plasmid expression system for the production of high titer retroviral vectors

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider