What question did this study set out to answer?

The aim is to develop a highly efficient transient expression system to produce high titer retroviral vectors.

January 1, 1995Nucleic Acids ResearchOpen Access

A transient three-plasmid expression system for the production of high titer retroviral vectors

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Authors

YSYuko SoneokaUniversity of Oxford PCPaula M. CannonUniversity of Southern California EREmma E. RamsdaleUniversity of Oxford

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Implication

Randomized trial demonstrates enhanced gene expression in human cells using a three-plasmid system, indicating potential for increased retroviral vector production.

Key Points

The aim is to develop a highly efficient transient expression system to produce high titer retroviral vectors.
Constructed MLV-based retroviral vectors with CMV promoter and SV40 origins of replication.
Used a highly transfectable human cell line with sodium butyrate to increase expression.
Separated gag-pol and env packaging components on distinct plasmids to minimize helper virus formation.
Achieved helper-free viral stocks of approximately 10^7 infectious units/ml.
Increased retroviral gene expression observed in SV40 T antigen carrying cell lines by 48 hours after transfection.

Cite This Study

Soneoka et al. (1995) studied this question.

synapsesocial.com/papers/69d8474752654bb436d18faf https://doi.org/10.1093/nar/23.4.628

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Implication

Randomized trial demonstrates enhanced gene expression in human cells using a three-plasmid system, indicating potential for increased retroviral vector production.

A transient three-plasmid expression system for the production of high titer retroviral vectors

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Member takes

Implication

Key Points

Cite This Study

A transient three-plasmid expression system for the production of high titer retroviral vectors

Discussion

Member takes

Implication

Key Points

Cite This Study

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