The A1 and A1B proteins of heterogeneous nuclear ribonucleoparticles modulate 5' splice site selection in vivo.

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Population

Mouse erythroleukemia (MEL) cell line (CB3C7) deficient in the expression of hnRNP A1

Comparison

Transient expression of A1 or A1B cDNA vs A1-expressing MEL cell lines and mutated A1 cDNA

Design

Preclinical

Authors

Discussion

Does not inform clinical practice; leaves open whether hnRNP A1 modulates splicing in human disease models.

Population

Mouse erythroleukemia (MEL) cell line (CB3C7) deficient in the expression of hnRNP A1

Intervention

Transient expression of A1 or A1B cDNA

Comparator

A1-expressing MEL cell lines and mutated A1 cDNA

Outcome

5' splice site selection on the adenovirus E1A pre-mRNAsurrogate

Demonstrates that in vivo variations in hnRNP A1 protein levels can influence 5' splice site selection.

Huang et al. (1994) studied this question.

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