What question did this study set out to answer?

Investigate the tissue distribution of the calcium-binding protein sorcin in normal cardiac tissue and evaluate its interaction with the cardiac ryanodine receptor.

November 1, 1995Journal of Biological ChemistryOpen Access

Association of Sorcin With the Cardiac Ryanodine Receptor

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Investigate the tissue distribution of the calcium-binding protein sorcin in normal cardiac tissue and evaluate its interaction with the cardiac ryanodine receptor.
Examined sorcin tissue distribution and subcellular localization in rodent cardiac myocytes using immunoelectron microscopy.
Performed reciprocal co-immunoprecipitation experiments with sorcin antiserum and ryanodine receptor antibodies in cardiac myocytes.
Transfected ryanodine receptor-negative Chinese hamster lung fibroblasts with sorcin and measured intracellular calcium changes following caffeine stimulation.
Sorcin localized to the sarcoplasmic reticulum of cardiac myocytes and reciprocally co-immunoprecipitated with the 565-kDa ryanodine receptor complex.
Forced sorcin expression in ryanodine receptor-negative fibroblasts prompted accumulation of ryanodine receptor protein and enabled rapid, transient intracellular calcium release upon caffeine exposure.

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Implication

Laboratory study demonstrates an interaction between sorcin and cardiac ryanodine receptors in rodent cells, suggesting a regulatory role in stabilizing calcium release channels.

Cite This Study

Meyers et al. (1995) studied this question.

synapsesocial.com/papers/69d9b7100d540cafc58372a8 https://doi.org/10.1074/jbc.270.44.26411

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Implication

Laboratory study demonstrates an interaction between sorcin and cardiac ryanodine receptors in rodent cells, suggesting a regulatory role in stabilizing calcium release channels.

Association of Sorcin With the Cardiac Ryanodine Receptor

Key Points

Discussion

Implication

Cite This Study

Association of Sorcin With the Cardiac Ryanodine Receptor

Key Points

Discussion

Implication

Cite This Study

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