Chemical decellularisation of porcine aortic valves alters the extracellular matrix, though seeded endothelial cells demonstrate the capacity to synthesize the lost components.
Hypothesis-generating for recellularised valve scaffolds; leaves open translation to clinical use.
Chemically induced decellularisation by Triton or Trypsine resulted in changes in the extracellular matrix constitution, which could lead to problems in valve functionality and cell growth and migration. Seeded endothelial cells were capable of synthesising extracellular matrix components lost by cell extraction. Further studies on tissue engineering should focus more on the effect of chemically induced cell extraction on the extracellular matrix of the remaining scaffold and the in vitro or in vivo replenishment of lost compounds.
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Grauss et al. (2005) studied this question.
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