Key result
Recellularization of porcine extracellular matrix with mesenchymal stem cells significantly reduced IL-1β and TNF-α production, attenuating the xenogenic immune response.
Why the study?
Structural valvular deterioration of xenogenic heart valve replacements is thought to be caused by a chronic immune response, prompting the development of porcine extracellular matrix that elicits minimal inflammatory immune response.
Does recellularization of decellularized porcine extracellular matrix with mesenchymal stem cells reduce the inflammatory immune response when exposed to human whole blood?
Does recellularization of decellularized porcine extracellular matrix with mesenchymal stem cells reduce the inflammatory immune response when exposed to human whole blood?
Recellularization of porcine extracellular matrix with mesenchymal stem cells attenuates the xenogenic immune response, suggesting potential as a scaffold for prosthetic heart valves.
Hypothesis-generating for recellularized porcine matrices as valve scaffolds; prospective in vivo studies needed before clinical translation.
Aim: Structural valvular deterioration of xenogenic heart valve replacements is thought to be due to a chronic immune response. We sought to engineer porcine extracellular matrix that elicits minimal inflammatory immune response. Materials & methods: Whole blood, bone marrow and pericardium were collected from patients undergoing elective cardiac surgery. Porcine extracellular matrix was decellularized, reseeded with homologous mesenchymal stem cells and exposed to whole blood. Results: DAPI stain confirmed the absence of cells after decellularization, and presence of mesenchymal stem cells after recellularization. There was a significant reduction in IL-1β and TNF-α production in the recellularized matrix. Conclusion: Recellularization of porcine matrix is successful at attenuating the xenogenic immune response and may provide a suitable scaffold to address the current limitations of prosthetic heart valve replacements.
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EL‐Andari et al. (2023) studied Patients undergoing elective cardiac surgery. Recellularized porcine extracellular matrix with homologous mesenchymal stem cells was evaluated on IL-1β and TNF-α production. Recellularization of porcine extracellular matrix with mesenchymal stem cells significantly reduced IL-1β and TNF-α production, attenuating the xenogenic immune response.
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