PulseTrendingJournal ClubResearchersJournalsExplore
Instagram
HomeTrendingJournal ClubExplore
Synapse
⌘+K
Synapse
January 1, 1990Nucleic Acids ResearchOpen Access

DNA recombination during PCR

View Full Paper
Ask AI
Bookmark
Share

Population

Two distinct HIV1 tat gene sequences (in vitro PCR model)

Comparison

6 fold increase in Taq DNA polymerase elongation… vs Standard Taq DNA polymerase elongation time

Design

Preclinical

Authors

AMAndreas MeyerhansJVJean‐Pierre VartanianSWSimon Wain–Hobson

Discussion

Loading...

Member takes

Overview

May reduce PCR recombination artifacts in genetic analyses; leaves open validation across heterogeneous samples.

Structured PICO

P
Population
Two distinct HIV1 tat gene sequences (in vitro PCR model)
I
Intervention
6 fold increase in Taq DNA polymerase elongation time
C
Comparator
Standard Taq DNA polymerase elongation time
O
Outcome
Frequency of recombinant DNA molecules

PCR co-amplification can lead to DNA recombination, which can be reduced by increasing Taq polymerase elongation time, highlighting a potential artifact in studying heterogeneous genetic material or a method to create chimeric molecules.

Cite This Study

Meyerhans et al. (1990) studied this question.

synapsesocial.com/papers/69dd1b7bceb896e1a0e52f87https://doi.org/10.1093/nar/18.7.1687
View Full Paper
Ask AI
Bookmark
Share