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November 2, 2023Blood

Pathogen Reduced Cryoprecipitated Fibrinogen Complex (IFC) and Cryoprecipitated AHF Contain Von Willebrand Factor with Comparable Binding to Collagen and Support Shear-Induced Platelet Thrombus Formation

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Authors

SYSubramanian YegneswaranCerus (United States)AZAlessandro ZarpellonScripps Research InstituteJOJennifer N. OrjeÇağ University

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Overview

In vitro comparative study shows pathogen-reduced fibrinogen complex matches conventional cryoprecipitate in shear-induced platelet adhesion, suggesting a viable alternative with longer shelf-life.

Key Points

  • To evaluate and compare the functional activity of von Willebrand factor (VWF) in pathogen-reduced cryoprecipitated fibrinogen complex (IFC) against conventional cryoprecipitated AHF (Cryo).
  • Assessed VWF multimeric composition and ristocetin-induced platelet aggregation using equimolar concentrations of VWF antigen from IFC and Cryo.
  • Reconstituted washed human red blood cells and platelets with Tyrode buffer spiked with Cryo or IFC (up to 1 U/mL VWF) and perfused them over fibrillar type I collagen across a shear rate gradient of 0 to 20,000 s⁻¹ in a Hele-Shaw flow chamber.
  • Visualized fluorescently labeled platelets to quantify total surface coverage, aggregate size, and aggregate density, including tests with monoclonal antibodies targeting VWF A1 and A3 domains.
  • IFC and conventional Cryo exhibited identical profiles of the largest high-molecular-weight VWF multimers and supported equivalent ristocetin-induced platelet aggregation.
  • Blood reconstituted with either IFC or Cryo supported platelet surface coverage across all tested shear rates aligned with native, unwashed whole blood controls.
  • Individual platelet aggregate sizes increased with rising shear stress, whereas targeted inhibition of VWF A1 and A3 domains markedly reduced platelet aggregate area and count.

Cite This Study

Yegneswaran et al. (2023) studied this question.

synapsesocial.com/papers/69dd397c0644c7b49d40c62bhttps://doi.org/10.1182/blood-2023-187124
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Retention of critical platelet hemostatic functions of amotosalen‐ UVA pathogen‐reduced cryoprecipitated fibrinogen complex2026
  2. 2Implementation and early outcomes with Pathogen Reduced Cryoprecipitated Fibrinogen Complex2024 · 6 citations
  3. 3Platelet Thrombus Formation on Collagen at High Shear Rates Is Mediated by von Willebrand Factor–Glycoprotein Ib Interaction and Inhibited by von Willebrand Factor–Glycoprotein IIb/IIIa Interaction2000 · 79 citations
  4. 4Adhesion of platelets to human artery subendothelium: effect of factor VIII-von Willebrand factor of various multimeric composition1984 · 134 citations
  5. 5Characterization of Collagen Thin Films for von Willebrand Factor Binding and Platelet Adhesion2011 · 38 citations