Why the study?
Does the newly developed FMDV serotyping lateral flow strip accurately detect and serotype FMDV compared to existing methods?
Does the newly developed FMDV serotyping lateral flow strip accurately detect and serotype FMDV compared to existing methods?
The newly developed lateral flow strip provides rapid, sensitive, and accurate simultaneous antigen detection and serotyping of foot-and-mouth disease virus without requiring extra devices.
May enable rapid on-site FMDV serotyping without extra equipment; leaves open need for prospective field validation before clinical adoption.
We developed a lateral flow strip using monoclonal antibodies (MAbs) which allows for rapid antigen detection and serotyping of foot-and-mouth disease virus (FMDV). This FMDV serotyping strip was able to detect all 7 serotypes and distinguish serotypes O, A, C and Asia1. Its sensitivities ranged from 10(3) to 10(4) of a 50% tissue culture infectious dose of each FMDV stain; this is equal to those of the commercial product Svanodip (Boehringer Ingelheim Svanova, Uppsala, Sweden), which can detect all seven serotypes of FMDV, but does not distinguish them. Our evaluation of the FMDV serotyping strip using a total of 118 clinical samples (vesicular fluids, vesicular epithelial emulsions and oral and/or nasal swabs) showed highly sensitive antigen detection and accuracy in serotyping in accordance with ELISA or RT-PCR. To the best of our knowledge, this is the first report on any FMDV serotyping strip that provides both rapid antigen detection and serotyping of FMDV at the same time on one strip without extra devices. This method will be useful in both FMD-free countries and FMD-infected countries, especially where laboratory diagnosis cannot be carried out.
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Morioka et al. (2015) studied this question.
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