What question did this study set out to answer?

To evaluate trypsin-induced alterations in the proteome and associated physiological changes in mammalian cells during routine subculture procedures.

May 11, 2010SHILAP Revista de lepidopterologíaOpen Access

Trypsin-induced proteome alteration during cell subculture in mammalian cells

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Authors

HHHsiang-Ling HuangLung Institute HHHsiang-Wei HsingNational Tsing Hua University TLTzu-Chia LaiNational Tsing Hua University

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Implication

Proteomic analysis reveals significant proteome alterations in mammalian cells following trypsinization, highlighting critical factors for in vitro experimental design.

Key Points

To evaluate trypsin-induced alterations in the proteome and associated physiological changes in mammalian cells during routine subculture procedures.
Conducted a comprehensive proteomic analysis to monitor cellular changes following standard trypsin-mediated cell detachment and subculturing.
Demonstrated that standard trypsinization induces substantial alterations in the mammalian cell proteome.
Identified distinct physiological perturbations caused by trypsin detachment that can influence downstream experimental outcomes.

Cite This Study

Huang et al. (2010) studied this question.

synapsesocial.com/papers/69dd599a80eea7d3f699bb5a https://doi.org/10.1186/1423-0127-17-36

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Implication

Proteomic analysis reveals significant proteome alterations in mammalian cells following trypsinization, highlighting critical factors for in vitro experimental design.

Trypsin-induced proteome alteration during cell subculture in mammalian cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Trypsin-induced proteome alteration during cell subculture in mammalian cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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