January 1, 2002Arteriosclerosis Thrombosis and Vascular Biology
Stromal Cells Are the Main Plasminogen Activator Inhibitor-1-Producing Cells in Human Fat
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Discussion
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Implication
Randomized trial compares PAI-1 localization in visceral and subcutaneous fats, indicating stromal cells’ key role.
Key Points
- The research aims to compare how plasminogen activator inhibitor-1 (PAI-1) is distributed in human visceral versus subcutaneous fat.
- Localized PAI-1 expression in visceral and subcutaneous fat using mRNA analysis and in vitro adipocyte differentiation
- Used real-time polymerase chain reaction to measure PAI-1 mRNA content in stromal and adipocyte fractions
- Applied in situ hybridization for PAI-1 mRNA detection in cultured adipocytes
- PAI-1 mRNA levels were 5-fold higher in the visceral versus subcutaneous stromal area (P=0.004)
- PAI-1 production is primarily derived from stromal cells, more prevalent in visceral fat
- PAI-1 secretion during adipocyte differentiation did not correlate with adipocyte maturation
Cite This Study
Bastelica et al. (2002) studied this question.