May 1, 1997Diabetes
Production of Plasminogen Activator Inhibitor 1 by Human Adipose Tissue: Possible Link Between Visceral Fat Accumulation and Vascular Disease
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Discussion
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Implication
Tissue analysis demonstrates higher plasminogen activator inhibitor 1 production by visceral fat than subcutaneous fat in humans, suggesting a direct link between central obesity and vascular disease.
Key Points
- To evaluate the expression and secretion of plasminogen activator inhibitor 1 (PAI-1) across cellular fractions of human adipose tissue, specifically comparing omental and subcutaneous fat depots.
- Assessed PAI-1 protein localization, mRNA expression, and antigen secretion in freshly isolated and cultured human stromal vascular cells, whole adipose tissue, and adipocyte fractions.
- Compared PAI-1 and leptin antigen production between paired omental and subcutaneous adipose tissue explants from the same individuals after 5 hours of incubation.
- Evaluated the regulatory effects of transforming growth factor-beta1 (TGF-β1), tumor necrosis factor-alpha (TNF-α), and cycloheximide on adipocyte PAI-1 production.
- PAI-1 mRNA and protein were identified in both stromal vascular and adipocyte fractions, with secreted antigen accumulation in conditioned medium being inhibited by cycloheximide.
- TGF-β1 significantly increased PAI-1 antigen production in the adipocyte fraction, whereas TNF-α had no significant effect.
- Omental tissue explants produced significantly more PAI-1 antigen than paired subcutaneous tissue after 5 hours of incubation, despite both territories exhibiting similar leptin production.
Cite This Study
Alessi et al. (1997) studied this question.