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April 16, 2026British Journal of Pharmacology

Study on the mechanism of the combined effects of high shear stress and antiplatelet drug on platelet haemostatic function

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Key result

Ticagrelor reduces shear-induced platelet activation in bovine blood, especially when given after shear exposure.

Why the study?

Blood pumps generate non-physiological shear stress (NPSS) that activates platelets and disrupts haemostasis, yet the effects of ticagrelor under NPSS remain unclear.

Does ticagrelor mitigate shear-induced platelet activation in bovine blood exposed to non-physiological shear stress?

Population

Citrated bovine blood circulated in a Rotaflow loop

Comparison

Ticagrelor before circulation vs after shear stress exposure vs varying shear stress levels

Design

In vitro experimental study

Follow-up

Up to 3 h

Authors

HWHongyu WangBeihang UniversityYLYuxia LiNingbo UniversityJJJinze JiaHenan Institute of Technology

Discussion

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Implication

May warrant ticagrelor timing optimization in MCS; leaves open clinical benefit pending human trials.

Key Points

  • The aim is to understand how non-physiological shear stress and ticagrelor affect platelet haemostatic function.
  • Citrated bovine blood circulated in a Rotaflow loop
  • Ticagrelor administered at different times relative to shear stress exposure
  • Flow cytometry and aggregometry used to measure platelet activation
  • Proteomics compared signaling pathways
  • Thromboelastography assessed clot kinetics.
  • Non-physiological shear stress increased platelet activation markers like P-selectin and decreased P2Y12 receptor expression.
  • Ticagrelor reduced shear-induced platelet activation and preserved P2Y12 receptor levels when administered post-shear.
  • Proteomics indicated lower Gi-coupled signaling after ticagrelor treatment.
  • Thromboelastography demonstrated faster clot formation with shear exposure moderated by ticagrelor.

Structured PICO

Does ticagrelor mitigate shear-induced platelet activation in bovine blood exposed to non-physiological shear stress?

P
Population
Citrated bovine blood circulated in a Rotaflow loop (500 ml, 5.0 l·min −1, Δ P of 0, 100 and 350 mmHg) for up to 3 h
I
Intervention
Ticagrelor (20 μM) administered either before circulation or after shear stress exposure
C
Comparator
No ticagrelor or different timing of administration under varying shear stress levels
O
Outcome
Platelet activation, P2Y12 receptor surface expression, adhesion, aggregation, signalling pathways (proteomics), and clot kinetics (TEG)surrogate

In an in vitro blood pump model, ticagrelor effectively reduced shear-induced platelet activation, particularly when administered after shear exposure, suggesting timing of antiplatelet therapy may optimize haemostasis in mechanical circulatory support.

Cite This Study

Wang et al. (2026) studied Platelet activation under non-physiological shear stress. Ticagrelor vs. Administration before circulation vs after shear stress exposure was evaluated on Platelet activation, P2Y12 receptor surface expression, adhesion, aggregation, and clot kinetics. Ticagrelor reduced shear-induced platelet activation in bovine blood, with the greatest reduction occurring when administered after shear exposure.

synapsesocial.com/papers/69e07d3c2f7e8953b7cbe39fhttps://doi.org/10.1111/bph.70433

Topics

Dual antiplatelet therapy
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Platelet-collagen interaction: is GPVI the central receptor?2003 · 1,164 citations
  2. 2Effect of pathological high shear exposure time on platelet activation and aggregation2023 · 13 citations
  3. 32017 ESC focused update on dual antiplatelet therapy in coronary artery disease developed in collaboration with EACTS2017 · 3,027 citations
  4. 4Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage2013 · 6 citations