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April 11, 2023Clinical Hemorheology and Microcirculation

Transient pathological high shear rate exposure significantly increased platelet activation and aggregation in a time-dependent manner compared to un-sheared whole blood.

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Why the study?

The effect of transiently elevated pathological high shear rates caused by vascular stenosis on platelet activation and aggregation function has not been clarified.

Population

Sodium citrate anticoagulated whole blood

Comparison

Pathological high shear rate exposure across varying times vs un-sheared blood with or without antiplatelet inhibitors

Design

In vitro microfluidic study

Key result

Transient pathological high shear rate exposure significantly increased platelet activation and aggregation in a time-dependent manner compared to un-sheared whole blood.

Authors

TZTiancong ZhangSichuan UniversityXHXiaojing HuangFujian Medical UniversityXGXuemei GaoWuhan No.1 Hospital

Discussion

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Member takes

Overview

Alerts to prothrombotic risk in high-shear settings; leaves open clinical translation pending in vivo studies.

Structured PICO

P
Population
Sodium citrate anticoagulated whole blood in an in vitro microfluidic chip model
E
Exposure
Exposure to pathological high shear rate (8302s-1) for varying times (3.16-25.3 ms) using microfluidic chips with 80% stenosis, with in vitro addition of aspirin, ticagrelor, tirofiban, or GP1BA
C
Comparator
Un-sheared whole blood
O
Outcome
Expression levels of platelet surface activation markers (P-selectin and GP IIb/IIIa) and degree of platelet aggregationsurrogate

Transient pathological high shear rate exposure induces platelet activation in a time-dependent manner, which can be differentially inhibited by various antiplatelet agents.

Cite This Study

Zhang et al. (2023) studied Platelet activation and aggregation under high shear stress. Pathological high shear rate (8302s-1) exposure vs. Un-sheared whole blood (physiological arterial shear rate 1500 s-1) was evaluated on Platelet activation (P-selectin and GP IIb/IIIa expression) and aggregation levels. Transient pathological high shear rate exposure significantly increased platelet activation and aggregation in a time-dependent manner compared to un-sheared whole blood.

synapsesocial.com/papers/6a706b05b27f1581782759cdhttps://doi.org/10.3233/ch-221567
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Constricted microfluidic devices to study the effects of transient high shear exposure on platelets2017 · 13 citations
  2. 2Duration of exposure to high fluid shear stress is critical in shear-induced platelet activation-aggregation2003 · 63 citations
  3. 3Evaluating the impact of transient shear stress on platelet activation, adhesion, and aggregation with microfluidic chip technique2023 · 18 citations
  4. 4Study on the mechanism of the combined effects of high shear stress and antiplatelet drug on platelet haemostatic function2026 · 1 citations
  5. 5Platelet Aggregation and Activation under Complex Patterns of Shear Stress2002 · 82 citations