The role of inositol 1,4,5‐trisphosphate receptors in Ca²⁺ signalling and the generation of arrhythmias in rat atrial myocytes
Population
Isolated rat atrial myocytes loaded with indo-1 and fluo-4
Comparison
Endothelin-1 and membrane-permeable InsP3 ester vs Cardiac glycosides, beta-adrenergic agonist, and…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
ET-1 arrhythmogenicity via InsP3 receptors in animal atrial myocytes is hypothesis-generating; human studies needed before therapeutic targeting.
Structured PICO
P
Population
Isolated rat atrial myocytes loaded with indo-1 and fluo-4
I
Intervention
Endothelin-1 (ET-1) and membrane-permeable InsP3 ester (InsP3 BM)
C
Comparator
Cardiac glycosides (digoxin), beta-adrenergic agonist (isoproterenol), and control conditions
O
Outcome
Arrhythmogenicity (arrhythmogenic index: ratio of number of premature extra Ca2+ transients over fold-increase in Ca2+ response)surrogate
The arrhythmogenic potency of endothelin-1 in atrial myocytes is driven by spatially specific recruitment of Ca2+ sparks via subsarcolemmal InsP3 receptors, highlighting a potential new target for atrial arrhythmia management.
Cite This Study
MacKenzie et al. (2002) studied this question.