August 1, 1995Diabetes
Hyperglycemia-Induced Thrombin Formation in Diabetes: The Possible Role of Oxidative Stress
Why the study?
Does intravenous glutathione prevent hyperglycemia-induced thrombin formation in type II diabetic patients and healthy subjects?
Population
16 subjects (9 type II diabetic patients and 7 healthy control subjects matched for age and body mass index)
Comparison
Oral glucose tolerance test, intravenous… vs Healthy controls; and comparison between…
Design
Other
Follow-up
180 minutes
Authors
Discussion
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Implication
GSH may blunt hyperglycemia-induced thrombin generation in diabetes; hypothesis-generating and requires randomized confirmation before practice change.
Key Points
- This study aims to explore the link between hyperglycemia, oxidative stress, and thrombin formation in diabetes.
- Evaluated 9 type II diabetic patients and 7 healthy controls matched for age and body mass index.
- Conducted oral glucose tolerance test (OGTT) and intravenous antioxidant glutathione (GSH) administration.
- Sample measurements were taken at specific intervals from -15 min to 180 min.
- During OGTT, prothrombin fragment 1 + 2 (F1+2) significantly increased in both groups.
- GSH administration during OGTT normalized F1+2 increases in both subjects.
- GSH alone resulted in a significant decrease in F1+2 in diabetic patients but showed no effect in healthy subjects.
Structured PICO
Does intravenous glutathione prevent hyperglycemia-induced thrombin formation in type II diabetic patients and healthy subjects?
P
Population
16 subjects (9 type II diabetic patients and 7 healthy control subjects matched for age and body mass index)
I
Intervention
Oral glucose tolerance test (OGTT), intravenous antioxidant glutathione (GSH) administration for 2 h, and OGTT plus intravenous GSH administration
C
Comparator
Healthy controls; and comparison between different experimental conditions (OGTT alone vs OGTT + GSH vs GSH alone)
O
Outcome
Prothrombin fragment 1 + 2 (F1+2) levels measured from 0 to 180 minutessurrogate
Intravenous glutathione prevents hyperglycemia-induced thrombin generation, suggesting oxidative stress mediates coagulation activation in diabetes.
Cite This Study
Ceriello et al. (1995) studied this question.