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Determine the kinetics, systemic dissemination, and extraintestinal tissue pathology associated with rotavirus viremia in a neonatal rat model.

April 26, 2006Journal of VirologyOpen Access

Rotavirus Viremia and Extraintestinal Viral Infection in the Neonatal Rat Model

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Determine the kinetics, systemic dissemination, and extraintestinal tissue pathology associated with rotavirus viremia in a neonatal rat model.
Inoculated 5-day-old rat pups intragastrically with simian rotavirus (RRV), human rotavirus (HAL1166), or control phosphate-buffered saline.
Assessed rotavirus antigen and infectious viral titers in blood and perfused organs using ELISA and fluorescent focus assays, alongside histological and immunohistochemical evaluations.
Viremia and systemic infection occurred at significantly lower viral titers than diarrhea, with an RRV 50% antigenemia dose of 1.8 x 10³ PFU compared to a 50% diarrhea dose of 7.7 x 10⁵ PFU.
Infectious rotavirus and antigens spread to multiple extraintestinal sites (stomach, intestines, liver, lungs, spleen, kidneys, pancreas, thymus, and bladder), with active replication identified in lung and vascular macrophages.
Infection caused acute liver and lung pathology, including bile duct inflammation, microsteatosis, and tissue necrosis, which persisted even in the presence of rotavirus-specific antibodies that prevented diarrhea.

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Implication

Animal study reveals systemic viremia and extraintestinal organ infection in neonatal rats, indicating rotavirus can cause widespread disease beyond the gastrointestinal tract.

Cite This Study

Crawford et al. (2006) studied this question.

synapsesocial.com/papers/6a0125b8f9e1acab462d79be https://doi.org/10.1128/jvi.80.10.4820-4832.2006

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Implication

Animal study reveals systemic viremia and extraintestinal organ infection in neonatal rats, indicating rotavirus can cause widespread disease beyond the gastrointestinal tract.

Rotavirus Viremia and Extraintestinal Viral Infection in the Neonatal Rat Model

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Discussion

Implication

Cite This Study

Rotavirus Viremia and Extraintestinal Viral Infection in the Neonatal Rat Model

Key Points

Discussion

Implication

Cite This Study

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