What question did this study set out to answer?

To determine whether solvent-free polysorbate 80 can effectively inactivate enveloped viruses in biopharmaceutical cell culture harvests and to identify its underlying virucidal mechanism.

December 17, 2019Biotechnology Progress

Insights into virus inactivation by polysorbate 80 in the absence of solvent

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Authors

DCDayue ChenNorthwestern University WLWen LuoChengdu University JHJacob HoffmanEastern Virginia Medical School

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Implication

Experimental study demonstrates enveloped virus inactivation by polysorbate 80 via enzymatic hydrolysis in cell harvest fluids, indicating a viable replacement for regulated Triton X-100.

Key Points

To determine whether solvent-free polysorbate 80 can effectively inactivate enveloped viruses in biopharmaceutical cell culture harvests and to identify its underlying virucidal mechanism.
Evaluated viral inactivation kinetics of xenotropic murine leukemia virus and pseudorabies virus using polysorbate 80 (PS80) in phosphate-buffered saline (PBS) and cell-free Chinese hamster ovary (CHO) cell harvests.
Tested the enzymatic hydrolysis mechanism in PBS by adding purified recombinant lysosomal phospholipase A2 isomer (rLPLA2) to generate virucidal free fatty acids.
Characterized virus log reduction factors across varying incubation times, temperatures, and surfactant concentrations, comparing PS80 and PS20 against standard Triton X-100 controls.
PS80 achieved robust viral inactivation in cell-free CHO bioreactor harvests comparable to Triton X-100, but exhibited no virucidal activity in pure PBS.
Supplementing PBS with rLPLA2 restored PS80 virucidal activity, confirming that host-cell esterases or lipases hydrolyze PS80 into virucidal oleic acid.
Virus inactivation kinetics by PS80 depended jointly on duration and temperature within operational ranges similar to Triton X-100, with polysorbate 20 exhibiting comparable activity.

Cite This Study

Chen et al. (2019) studied this question.

synapsesocial.com/papers/6a013a294716aad0cc85fc05 https://doi.org/10.1002/btpr.2953

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Implication

Experimental study demonstrates enveloped virus inactivation by polysorbate 80 via enzymatic hydrolysis in cell harvest fluids, indicating a viable replacement for regulated Triton X-100.

Insights into virus inactivation by polysorbate 80 in the absence of solvent

Discussion

Member takes

Implication

Key Points

Cite This Study

Insights into virus inactivation by polysorbate 80 in the absence of solvent

Discussion

Member takes

Implication

Key Points

Cite This Study

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