Improved quantification of a commercial enzyme-linked immunosorbent assay kit for measuring anti-MDA5 antibody

OBJECTIVES: anti-MDA5 test. METHODS: anti-MDA5 test side-by-side. For the commercial ELISA kit, serum samples diluted 1:101 were used according to the manufacturer's protocol, but serial dilutions of sera were also examined to identify the optimal serum dilution for quantification. RESULTS: anti-MDA5 test, including a better correlation with the in-house ELISA results and an increased sensitivity to change. CONCLUSION: We improved the ability of the commercial ELISA kit to quantify anti-MDA5 antibody titer by altering its protocol.