Garland Rolling Circle Amplification Mediated Self-Priming Extension Strategy for Sensitive and Label-Free Pseudomonas aeruginosa Analysis in Perioperative Period

CFU/mL. The assay exhibits excellent specificity against non-target bacteria, robust stability during storage, and satisfactory anti-interference capability in complex clinical matrices. Validation using clinical samples demonstrates excellent agreement with the gold-standard colony counting method while reducing the assay time to less than 2.5 h without requiring nucleic acid extraction or thermal cycling. With its label-free design, isothermal amplification, and operational simplicity, this strategy holds great promise for point-of-care testing in perioperative settings and can be readily adapted for detecting other pathogens by substituting the corresponding aptamer.