Acute nitric oxide synthase inhibition induces greater increases in blood pressure in female versus male Wistar Kyoto rats

Figures

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  FIGURE 1. NOS inhibition results in dose dependent increases in BP in male and female WKY. BP responses to graded bolus doses of the nonspecific NOS inhibitor L‐NAME (0.08–2.5 mg/kg, i.v) (a) or the specific NOS1 inhibitor VNIO (0.32–10.0 mg/kg, iv) (b) in male and female WKY. Data are expressed as mean ± SEM; n = 4–5 in each group. *Indicates p < 0.05 versus corresponding male WKY baseline and ≠ indicates p < 0.05 versus corresponding female WKY baseline.

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  FIGURE 2. Female WKY are more sensitive to L‐NAME induced increases in BP versus males. BP prior to and during L‐NAME infusion (1.0 mg/kg/min, i.v) for 60 min in anesthetized male and female WKY (a). All data are expressed as mean ± SEM; n = 5–6 in each group * indicates p < 0.05 versus baseline values of either male or female WKY and ≠ indicates p < 0.05 versus baseline female WKY. Percent change in BP from baseline values in male and female WKY after 30‐min and 60‐min infusion of L‐NAME (b). λ Indicates p < 0.05 versus male WKY after 30 min of L‐NAME infusion. Urine volume (c) and glomerular filtration rate (GFR, d) prior to and during acute L‐NAME infusion (1.0 mg/kg/min, i.v) for 60 min in anesthetized male and female WKY. *Indicates p < 0.05 versus baseline male or female WKY and ≠ indicates p < 0.05 versus 30 infusion of L‐NAME in male or female WKY.

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  FIGURE 3. Selective NOS1 inhibition increases BP similarly in male and female WKY. BP prior to and during VNIO infusion (0.5 mg/kg/min, i.v) for 60 min in anesthetized male and female WKY (a). All data are expressed as mean ± SEM; n = 5–6 in each group. * Indicates p < 0.05 versus baseline values of either male or female WKY and ≠ indicates p < 0.05 versus baseline female WKY. Percent change in BP from baseline values in male and female WKY after 30 min and 60 min infusion of VNIO (b). λ Indicates p < 0.05 versus male WKY after 30 min of VNIO infusion. Urine volume (c) and glomerular filtration rate (GFR, d) prior to and during VNIO infusion (0.5 mg/kg/min, i.v) for 60 min in anesthetized male and female WKY. * Indicates p < 0.05 versus baseline male or female WKY and ≠ indicates p < 0.05 versus 30 infusion of VNIO in male or female WKY.