What question did this study set out to answer?

To elucidate the molecular mechanism by which the Pan2-Pan3 deadenylase complex recognizes poly(A) ribonucleoprotein complexes to initiate poly(A) tail degradation.

May 1, 2019CellOpen Access

Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase

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Authors

ISIngmar B. SchäferDeutsches Diabetes-Zentrum e.V.MYMasami YamashitaTokyo Institute of Technology JSJan M. SchullerPhilipps University of Marburg

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Implication

Structural analysis reveals how Pan2-Pan3 deadenylase recognizes poly(A) ribonucleoprotein complexes, indicating how repeating protein units regulate mRNA tail length and stability.

Key Points

To elucidate the molecular mechanism by which the Pan2-Pan3 deadenylase complex recognizes poly(A) ribonucleoprotein complexes to initiate poly(A) tail degradation.
Reconstituted mRNA poly(A) tail degradation in vitro using purified recombinant proteins including Pan2-Pan3 and Pab1.
Determined the cryo-electron microscopy (cryo-EM) structure of Pan2-Pan3 bound to a poly(A) RNP composed of 90 adenosines and three Pab1 protomers.
Pan2-Pan3 selectively associates with and deadenylates poly(A) RNPs containing two or more Pab1 protomers.
Structural analysis shows conserved features of Pan2-Pan3 engage Pab1 oligomer interfaces to thread the poly(A) RNA substrate into the nuclease active site, acting as a molecular ruler for tail length.

Cite This Study

Schäfer et al. (2019) studied this question.

synapsesocial.com/papers/6a0cb81a85c3cfd5d2eb1ee3 https://doi.org/10.1016/j.cell.2019.04.013

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Implication

Structural analysis reveals how Pan2-Pan3 deadenylase recognizes poly(A) ribonucleoprotein complexes, indicating how repeating protein units regulate mRNA tail length and stability.

Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase

Discussion

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Implication

Key Points

Cite This Study

Molecular Basis for poly(A) RNP Architecture and Recognition by the Pan2-Pan3 Deadenylase

Discussion

Member takes

Implication

Key Points

Cite This Study

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