Single-cell origin of mouse hemopoietic colonies expressing multiple lineages in variable combinations.
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Discussion
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Implication
In vitro single-cell assay demonstrates diverse multilineage differentiation in mouse hemopoietic progenitors, suggesting progressive and stochastic lineage restriction.
Key Points
- To determine whether multilineage hemopoietic colonies originate from individual cells and to evaluate the patterns of lineage commitment in multipotential progenitors.
- Transferred single cells isolated from blast cell colonies into individual culture dishes using a micromanipulator for secondary colony formation.
- Assessed the cellular composition of mature colonies using May-Grunwald-Giemsa staining and performed replating assays to test for unexpressed developmental potentials across 50 mixed colonies.
- Identified 50 mixed colonies featuring seven distinct two-lineage combinations, six three-lineage combinations, and multiple colonies with more than three distinct cell lineages.
- Replating experiments confirmed that progenitors were terminally committed to the specific lineages revealed by morphological staining, supporting progressive and stochastic lineage restriction.
Cite This Study
Suda et al. (1983) studied this question.