What question did this study set out to answer?

To characterize the expression pattern of mesothelin across developmental stages and evaluate its biological necessity in vivo by creating mesothelin-null mice.

April 1, 2000Molecular and Cellular BiologyOpen Access

Mesothelin Is Not Required for Normal Mouse Development or Reproduction

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To characterize the expression pattern of mesothelin across developmental stages and evaluate its biological necessity in vivo by creating mesothelin-null mice.
Assessed mesothelin mRNA expression in embryonic stages (E 7.0, E 15.0, E 17.0) and adult tissues via Northern hybridization.
Generated knockout mice by targeted replacement of the mesothelin gene with a neomycin resistance gene cassette.
Mesothelin transcripts measuring 2.5-kb were present in embryonic stages and widely distributed in adult lung, heart, spleen, liver, kidney, and testis tissues.
Homozygous mutant mice lacked detectable mesothelin mRNA and protein, conformed to expected Mendelian inheritance ratios, and showed normal male and female reproductive capacity.
Histological and gross anatomical inspections revealed no structural defects across tissues that normally express mesothelin.

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Implication

Gene knockout study demonstrates normal development and fertility in mesothelin-deficient mice, indicating the glycoprotein is nonessential for baseline physiological function.

Cite This Study

Bera et al. (2000) studied this question.

synapsesocial.com/papers/6a0d02387e512f50ffcc9541 https://doi.org/10.1128/mcb.20.8.2902-2906.2000

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Implication

Gene knockout study demonstrates normal development and fertility in mesothelin-deficient mice, indicating the glycoprotein is nonessential for baseline physiological function.

Mesothelin Is Not Required for Normal Mouse Development or Reproduction

Key Points

Discussion

Implication

Cite This Study

Mesothelin Is Not Required for Normal Mouse Development or Reproduction

Key Points

Discussion

Implication

Cite This Study

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