What question did this study set out to answer?

To examine whether nitric oxide and interleukin-1 beta act synergistically to regulate cyclooxygenase-2 expression and prostaglandin E2 production in glomerular mesangial cells.

May 1, 1996Journal of Clinical InvestigationOpen Access

Nitric oxide amplifies interleukin 1-induced cyclooxygenase-2 expression in rat mesangial cells.

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Authors

TTT. TetsukaHitachi High-Tech (Japan)DDDorit Daphna-IkenWashington University in St. Louis BMBrent W. MillerIndiana University Bloomington

Discussion

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Implication

In vitro study demonstrates that nitric oxide amplifies interleukin-1-induced COX-2 expression in rat mesangial cells, highlighting a synergistic pathway promoting glomerular inflammation.

Key Points

To examine whether nitric oxide and interleukin-1 beta act synergistically to regulate cyclooxygenase-2 expression and prostaglandin E2 production in glomerular mesangial cells.
Treated cultured rat mesangial cells with interleukin-1 beta alone or in combination with nitric oxide donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine.
Modulated cyclic GMP signaling pathways using the soluble guanylate cyclase inhibitor methylene blue, the cyclic GMP analogue 8-Br-cGMP, and atrial natriuretic peptide to assess regulatory mechanisms.
Nitric oxide donors alone exerted no effect on basal prostaglandin E2 synthesis or cyclooxygenase-2 mRNA and protein levels, but markedly amplified interleukin-1 beta-induced expression of both markers.
The nitric oxide-mediated increase in cyclooxygenase-2 mRNA was reversed by methylene blue and reproduced by both 8-Br-cGMP and atrial natriuretic peptide, indicating cGMP dependence.

Cite This Study

Tetsuka et al. (1996) studied this question.

synapsesocial.com/papers/6a0d43ea48a82a5ce309adb0 https://doi.org/10.1172/jci118641

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Implication

In vitro study demonstrates that nitric oxide amplifies interleukin-1-induced COX-2 expression in rat mesangial cells, highlighting a synergistic pathway promoting glomerular inflammation.

Nitric oxide amplifies interleukin 1-induced cyclooxygenase-2 expression in rat mesangial cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

Nitric oxide amplifies interleukin 1-induced cyclooxygenase-2 expression in rat mesangial cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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