Key result
Renin activation of the prorenin receptor on human immune cells induces ERK1/2, IL-6, and COX-2.
The (pro)renin receptor is expressed in human inflammatory cells and mediates renin-induced inflammatory responses, suggesting a potential role in the pathophysiology of ANCA-associated glomerulonephritis.
Identifies a novel inflammatory pathway in ANCA-associated glomerulonephritis that should not yet.
The renin-angiotensin system (RAS) is involved in inflammation. The signaling via the ANG II type 1 receptor in human lymphocytes and monocytes, which play key roles in pathophysiology of glomerulonephritis (GN), can enhance inflammation. However, the role of the (pro)renin receptor [(P)RR], a component of the RAS, in inflammatory reactions is unknown. We assessed whether (P)RR is expressed in human lymphocytes and monocytes by RT-PCR, Western blotting, flow cytometry, and immunohistochemistry, and whether (P)RR functions in inflammation. (P)RR mRNA and protein were expressed in human peripheral blood mononuclear cells (PBMCs). Flow cytometric analysis revealed high expression of (P)RR on monocytes. (P)RR was present on PBMCs, infiltrating lymphocytes, and macrophages around glomeruli with a crescent in anti-neutrophil cytoplasmic antibody (ANCA)-associated GN. Renin stimulation of PBMCs from healthy subjects in the presence of the ANG II type 1 receptor and ANG II type 2 receptor blockers induced ERK1/2 phosphorylation and release of IL-6 and expression of cyclooxygenase-2 (COX-2). The increases in cytokine release and COX-2 expression were inhibited in the presence of an ERK1/2 inhibitor. (P)RR knockdown by small interfering RNA in U937 cells, a human leukemic monocyte lymphoma cell line, significantly decreased ERK1/2 phosphorylation after renin stimulation. Thus (P)RR expressed in human inflammatory cells might contribute to inflammation in ANCA-associated GN.
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Narumi et al. (2014) studied ANCA-associated glomerulonephritis (GN). Renin stimulation and (P)RR knockdown was evaluated on (P)RR expression and function (ERK1/2 phosphorylation, IL-6 release, COX-2 expression). The (pro)renin receptor is expressed on human monocytes and lymphocytes, and its activation by renin induces ERK1/2 phosphorylation, IL-6 release, and COX-2 expression.
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