Why the study?
The mouse model plays a central role in deciphering post-infarct healing mechanisms, prompting the need to comprehensively assess in-vivo remodeling using native MRI across prominent infarct models.
A native CINE CMR protocol using multi-slice sector-based analysis provides a versatile and accessible tool for comprehensively assessing post-infarct LV remodeling in mouse models.
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Mouse MI model yields marked LV dilation; leaves open clinical translation while extending accessible CINE CMR for preclinical remodeling studies.
Novel therapeutic strategies aiming at improving the healing process after an acute myocardial infarction are currently under intense investigation. The mouse model plays a central role for deciphering the underlying mechanisms on a molecular and cellular level. Therefore, we intended to assess in-vivo post-infarct remodeling as comprehensively as possible using an expedient native magnetic resonance imaging (MRI) in the two most prominent infarct models, permanent ligation (PL) of the left anterior descending artery (LAD) versus ischemia reperfusion (I/R). Mice were subjected to either permanent or transient (45 min) occlusion of the LAD. After 3 weeks, examinations were performed with a 7-Tesla small animal MRI system. Data analysis was performed with the freely available software Segment. PL resulted in a massive dilation of the left ventricle, accompanied by hypertrophy of the non-infarcted myocardium and a decline of contractile function. These effects were less pronounced following I/R compared to healthy animals. Single plane assessments were not sufficient to capture the specific differences of left ventricular (LV) properties between the two infarct models. Bulls-eye plots were found to be an ideal tool for qualitative LV wall assessment, whereas a multi-slice sector-based analysis of wall regions is ideal to determine differences in hypertrophy, lateral wall thinning and wall thickening on a quantitative level. We combine the use of polar map-based analysis of LV wall properties with volumetric measurements using simple CINE CMR imaging. Our strategy represents a versatile and easily available tool for serial assessment of the LV during the remodeling process. Our study contributes to a better understanding of the effects of novel therapies targeting the healing of damaged myocardium.
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Lang et al. (2021) studied Myocardial infarction (n=19). Permanent ligation (PL) of the left anterior descending artery vs. Healthy control animals was evaluated on End-diastolic volume (EDV) (p=<0.001).
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