Key result
Feline calicivirus maintains wild-type growth kinetics despite gross changes in VP2 expression levels.
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Feline calicivirus can tolerate significant changes in the expression levels of its minor capsid protein VP2 without affecting viral growth kinetics.
Caliciviruses use reinitiation of translation governed by a 'termination upstream ribosomal binding site' (TURBS) for expression of their minor capsid protein VP2. Mutation analysis allowed to identify sequences surrounding the translational start/stop site of the feline calicivirus (FCV) that fine tune reinitiation frequency. A selection of these changes was introduced into the infectious FCV cDNA clone to check the influence of altered VP2 levels on virus replication. In addition, full length constructs were established that displayed a conformation, in which VP2 expression occurred under control of a duplicated subgenomic promoter. Viable viruses recovered from such constructs revealed a rather broad range of VP2 expression levels but comparable growth kinetics showing that caliciviruses can tolerate gross changes of the VP2 expression level.
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Haß et al. (2014) studied Feline Calicivirus. Altered VP2 expression levels vs. Wild-type FCV was evaluated on Virus recovery and growth kinetics. Feline calicivirus can tolerate gross changes in minor capsid protein VP2 expression levels, with viable viruses showing comparable growth kinetics to wild-type despite altered VP2 levels.
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