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October 27, 2011AJP Regulatory Integrative and Comparative PhysiologyOpen Access

Transpulmonary lactate shuttle

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Key result

Epinephrine stimulation significantly decreased tracer-measured fractional extraction of lactate to -25.3% compared to 16.6% in controls, indicating transpulmonary lactate production.

Population

Anesthetized rat model

Comparison

Lactate load and epinephrine stimulation with… vs Control condition

Design

Preclinical

Authors

MJMatthew L. JohnsonCEChi‐An W. EmhoffMHMichael A. Horning

Discussion

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Overview

Pulmonary AV lactate gradients may miss parenchymal production during epinephrine; leaves open accurate assessment of lung lactate metabolism.

Structured PICO

P
Population
Anesthetized rat model
I
Intervention
Lactate load (via lactate clamp) and epinephrine stimulation with primed-continuous infusion of [U-(13)C]lactate
C
Comparator
Control condition
O
Outcome
Transpulmonary lactate kinetics (uptake, concentrations, gradient, and fractional extractions)surrogate

Main Result

Absolute Event Rate: -25.3% vs 16.6%

p-value: p=<0.05

Pulmonary arterial-vein concentration difference measurements across the lungs provide an incomplete picture of parenchymal lactate metabolism, particularly during epinephrine stimulation.

Limitations

  • Cardiac output was estimated from literature rather than measured directly
  • Anesthesia may have raised baseline arterial lactate levels
  • Tracer infusion was limited to 1 hour

Cite This Study

Johnson et al. (2011) studied Healthy (animal model) (n=21). Epinephrine stimulation and Lactate clamp vs. Control (tracer 90 µg/kg/min) was evaluated on Tracer-measured fractional extraction of lactate (FEXTM) during epinephrine stimulation (p=<0.05). Epinephrine stimulation significantly decreased tracer-measured fractional extraction of lactate to -25.3% compared to 16.6% in controls, indicating transpulmonary lactate production.

synapsesocial.com/papers/6a0ee5f6b7cc3b883f22dd49https://doi.org/10.1152/ajpregu.00402.2011
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Also Consider

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  1. 1Evidence of separate pathways for lactate uptake and release by the perfused rat heart2001 · 93 citations
  2. 2Lactate, pyruvate, glucose, and free fatty acid in mixed venous and arterial blood1963 · 25 citations
  3. 3Arteriovenous differences of lactate and pyruvate across healthy and diseased human lung.1973 · 17 citations
  4. 4Hypoxia-Inducible Factor 2α Plays a Critical Role in the Formation of Alveoli and Surfactant2011 · 45 citations
  5. 5Evaluation of the isotopic equilibration between lactate and pyruvate1988 · 56 citations