Key result
The addition of a 10-fold molar excess of the NS4A peptide 4A18-40 increased the proteolytic efficiency of hepatitis C virus NS3 protease by approximately 40 times.
Population
in vitro NS3 protease assay system consisting of recombinant MBP-NS3 fusion protein, synthetic NS4A…
Design
Preclinical
Authors
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May guide NS3-NS4A inhibitor design for HCV; leaves open antiviral efficacy in vivo.
Effect estimate: increased by about 40 times
Identification of the core sequence of NS4A required for enhancing NS3 protease activity provides a potential novel strategy for inhibiting hepatitis C virus proliferation.
Shimizu et al. (1996) studied Hepatitis C virus. NS4A fragments (4A18-40) was evaluated on NS3 protease activity (kcat/Km) (increased by about 40 times). The addition of a 10-fold molar excess of the NS4A peptide 4A18-40 increased the proteolytic efficiency of hepatitis C virus NS3 protease by approximately 40 times.
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