Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 1, 1992Circulation Research

Gap junctional conductance in ventricular myocyte pairs isolated from postischemic rabbit myocardium.

View Full Paper
Ask AI
Bookmark
Share

Key result

Postischemic rabbit ventricular myocyte pairs had a significantly greater proportion of poorly communicating cells compared to controls (8 of 15 vs 0 of 15 and 1 of 13; p<0.02).

Why the study?

Does ischemia and reperfusion affect gap junctional conductance in isolated rabbit ventricular myocytes?

Population

Ventricular myocyte pairs isolated from Langendorff-perfused rabbit hearts

Comparison

30 minutes of global normothermic ischemia… vs 75 minutes of control perfusion, or immediately…

Design

Preclinical

Authors

RKRobert S. KievalJSJ F SpearEME N Moore

Discussion

Loading...

Member takes

Overview

May implicate gap junction heterogeneity in reperfusion arrhythmias; leaves open translation beyond this rabbit model.

Key Points

  • To investigate the effects of ischemia and reperfusion on gap junctional conductance between ventricular myocytes.
  • Isolated ventricular myocyte pairs from rabbit hearts after varying ischemic conditions.
  • Used whole-cell recording techniques to measure gap junctional conductance (gj).
  • Compared data from myocytes isolated after ischemia, control perfusion, and immediately after heart removal.
  • Mean gap junctional conductance was similar across all groups (0.88 ± 0.27, 1.15 ± 0.18, and 1.24 ± 0.25 microS; p > 0.05).
  • The postischemic group showed a greater proportion of poorly communicating cell pairs (gj < 25% in 8 of 15 pairs; p < 0.02).
  • Indicates that postischemic myocytes have heterogeneous coupling despite normal mean gj, potentially increasing arrhythmogenic risk.

Structured PICO

Does ischemia and reperfusion affect gap junctional conductance in isolated rabbit ventricular myocytes?

P
Population
Ventricular myocyte pairs isolated from Langendorff-perfused rabbit hearts
I
Intervention
30 minutes of global normothermic ischemia followed by 30 minutes of reperfusion
C
Comparator
75 minutes of control perfusion, or immediately after removal of the heart
O
Outcome
Gap junctional conductance (gj) between isolated ventricular myocytessurrogate

Main Result

Absolute Event Rate: 53.3% vs 0%

p-value: p=<0.02

Postischemic rabbit ventricular myocyte pairs exhibit heterogeneous gap junctional conductance with a significant proportion of poorly communicating cells, which may contribute to reperfusion-induced arrhythmias.

Cite This Study

Kieval et al. (1992) studied Myocardial ischemia and reperfusion (n=43). Global normothermic ischemia and reperfusion vs. Control perfusion or immediate removal was evaluated on Proportion of poorly communicating cell pairs (gap junctional conductance <25% of mean) (p=<0.02). Postischemic rabbit ventricular myocyte pairs had a significantly greater proportion of poorly communicating cells compared to controls (8 of 15 vs 0 of 15 and 1 of 13; p<0.02).

synapsesocial.com/papers/6a0f97f492676d5461fcdcbchttps://doi.org/10.1161/01.res.71.1.127
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Acute ischaemia and gap junction modulation modify propagation patterns across Purkinje-myocardial junctions2025 · 4 citations
  2. 2Myocardial gap junction organization in ischemia and infarction1995 · 52 citations
  3. 3Gap junction-mediated spread of cell injury and death during myocardial ischemia–reperfusion2004 · 153 citations
  4. 4Characterization of Gap Junction Channels in Adult Rabbit Atrial and Ventricular Myocardium1997 · 145 citations
  5. 5Persistence of gap junction communication during myocardial ischemia2001 · 71 citations