Extracellular acidosis and increased extracellular potassium decreased resting membrane potential, Vmax, and slowed conduction in guinea pig ventricular muscle, mimicking acute ischemia.
Does extracellular acidosis combined with increased extracellular potassium alter action potential characteristics and conduction in guinea pig ventricular muscle?
The combined effects of extracellular acidosis and increased extracellular potassium mimic many known electrophysiological effects of acute ischemia in ventricular fibers.
We studied the individual and combined effects of extracellular acidosis and increases in extracellular potassium on action potential characteristics and conduction in order to gain a better understanding of the effects of acute ischemia. At each level of potassium between 2.7 and 17 mm, acidosis induced by increasing Pco2 (respiratory acidosis) and by decreasing HCO3- (metabolic acidosis) decreased resting membrane potential, the maximum rate of rise of the action potential upstroke (Vmax), and slowed conduction. Metabolic acidosis consistently and significantly lengthened the steady state action potential duration whereas respiratory acidosis did not. Respiratory acidosis caused changes in resting membrane potential, Vmax, and conduction velocity; which occurred more rapidly and were of greater magnitude than the changes induced by metabolic acidosis. The changes in Vmax induced both types of acidosis were due to a change in the resting membrane potential-Vmax relationship as well as to the changes in the resting membrane potential. The conduction slowing induced by acidosis was greater when potassium was 9 and 13 mM than when potassium was 5.4 mm. Our results suggest that acidosis causes important changes in the electrophysiological properties of ventricular fibers and that many of the known electrophysiological effects of acute ischemia can be mimicked by the combined effects of extracellular acidosis and an increase in extracellular potassium.
Kagiyama et al. (1982) studied Acute ischemia (simulated). Extracellular acidosis and increased extracellular potassium vs. Different levels of potassium and acidosis was evaluated on Action potential characteristics and conduction velocity. Extracellular acidosis and increased extracellular potassium decreased resting membrane potential, Vmax, and slowed conduction in guinea pig ventricular muscle, mimicking acute ischemia.