What question did this study set out to answer?

To analyze the regulation of CD27 expression across subsets of mature T-lymphocytes and determine whether the loss of CD27 represents an irreversible marker of T-cell differentiation.

September 1, 1993The Journal of ImmunologyOpen Access

Regulation of CD27 expression on subsets of mature T-lymphocytes.

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Authors

RHRogier Q. HintzenLeiden University Medical Center RJRolien de JongWageningen University & Research SLSusanne M.A. LensUtrecht University

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Implication

Laboratory study reveals irreversible loss of CD27 following activation in human T-cell subsets, indicating that CD27 loss marks stable differentiation rather than temporary activation.

Key Points

To analyze the regulation of CD27 expression across subsets of mature T-lymphocytes and determine whether the loss of CD27 represents an irreversible marker of T-cell differentiation.
Analyzed CD27 surface expression and soluble release across peripheral blood CD4+ and CD8+ T-cell subsets distinguished by CD45RA and CD45RO expression.
Stimulated purified T-cell subsets and long-term T-cell clones with anti-CD3 monoclonal antibodies to assess CD27 protein reinduction and mRNA levels in comparison with FAS/APO-1.
Stimulation with anti-CD3 monoclonal antibodies up-regulated CD27 membrane expression and soluble release across CD4+ and CD8+ subsets, with preferential membrane up-regulation on CD45RO- cells over CD45RA- cells.
Stimulation failed to reinduce CD27 expression at either the protein or mRNA level in CD4+CD45RA-CD27- T cells and long-term T-cell clones, confirming that down-regulation is irreversible and distinct from the regulation of homologous receptor FAS/APO-1.

Cite This Study

Hintzen et al. (1993) studied this question.

synapsesocial.com/papers/6a0fb66b2badbc352afe96e4 https://doi.org/10.4049/jimmunol.151.5.2426

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Implication

Laboratory study reveals irreversible loss of CD27 following activation in human T-cell subsets, indicating that CD27 loss marks stable differentiation rather than temporary activation.

Regulation of CD27 expression on subsets of mature T-lymphocytes.

Discussion

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Implication

Key Points

Cite This Study

Regulation of CD27 expression on subsets of mature T-lymphocytes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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