What question did this study set out to answer?

To establish an in vitro methodology for quantifying intracellular pH in ascites tumor cells and analyze how alterations in external buffer conditions and carbon dioxide tension affect internal cell acidity.

April 1, 1964JNCI Journal of the National Cancer Institute

Intracellular pH of the Ehrlich Ascites Tumor Cell2

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Authors

DPDoris T. PooleUniversity of North Carolina at Chapel Hill TBThomas ButlerAlbert Einstein College of Medicine WWWilliam J. WaddellMathematica Policy Research

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Implication

In vitro study demonstrates regulation of intracellular pH in Ehrlich ascites tumor cells under altered buffer environments, highlighting cellular acid-base adaptation mechanisms.

Key Points

To establish an in vitro methodology for quantifying intracellular pH in ascites tumor cells and analyze how alterations in external buffer conditions and carbon dioxide tension affect internal cell acidity.
Determined intracellular pH in vitro using the partition equilibrium of the radiolabeled weak acid 5,5-dimethyl-2,4-oxazolidinedione-2-C14 across cellular membranes.
Quantified extracellular fluid volume in cell preparations utilizing inulin-carboxyl-C14.
Assessed Ehrlich ascites tumor cell internal pH under varied carbon dioxide tension in bicarbonate buffer, variable external pH at fixed carbon dioxide tension, and varying pH in phosphate buffer.
Validated a radiolabeled weak-acid distribution method combined with extracellular fluid space tracing for reliable measurement of intracellular pH in tumor cell suspensions.
Observed systematic shifts in Ehrlich ascites tumor cell intracellular pH following alterations in external carbon dioxide tension and buffer pH in both bicarbonate and phosphate systems.

Cite This Study

Poole et al. (1964) studied this question.

synapsesocial.com/papers/6a0ff845d8c5cf602efd70b2 https://doi.org/10.1093/jnci/32.4.939

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April 1, 1964JNCI Journal of the National Cancer Institute

Intracellular pH of the Ehrlich Ascites Tumor Cell2

View Full Paper

Ask AI

Bookmark

Authors

DPDoris T. PooleUniversity of North Carolina at Chapel Hill TBThomas ButlerAlbert Einstein College of Medicine WWWilliam J. WaddellMathematica Policy Research

Discussion

Member takes

Implication

In vitro study demonstrates regulation of intracellular pH in Ehrlich ascites tumor cells under altered buffer environments, highlighting cellular acid-base adaptation mechanisms.

Key Points

To establish an in vitro methodology for quantifying intracellular pH in ascites tumor cells and analyze how alterations in external buffer conditions and carbon dioxide tension affect internal cell acidity.
Determined intracellular pH in vitro using the partition equilibrium of the radiolabeled weak acid 5,5-dimethyl-2,4-oxazolidinedione-2-C14 across cellular membranes.
Quantified extracellular fluid volume in cell preparations utilizing inulin-carboxyl-C14.
Assessed Ehrlich ascites tumor cell internal pH under varied carbon dioxide tension in bicarbonate buffer, variable external pH at fixed carbon dioxide tension, and varying pH in phosphate buffer.
Validated a radiolabeled weak-acid distribution method combined with extracellular fluid space tracing for reliable measurement of intracellular pH in tumor cell suspensions.
Observed systematic shifts in Ehrlich ascites tumor cell intracellular pH following alterations in external carbon dioxide tension and buffer pH in both bicarbonate and phosphate systems.

Cite This Study

Poole et al. (1964) studied this question.

synapsesocial.com/papers/6a0ff845d8c5cf602efd70b2 https://doi.org/10.1093/jnci/32.4.939

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