Key result
Pressure overload induced by abdominal aortic banding in rats increased biglycan mRNA expression up to 2.88-fold in myocardial interstitial and capillary cells compared to sham operation.
Biglycan mRNA expression is significantly increased in mesenchymal, endothelial, and smooth muscle cells of pressure-overloaded rat hearts, suggesting it contributes to ventricular and vascular remodeling.
Answers are grounded in this paper and cite their sources.
Biglycan upregulation may mediate remodeling in overloaded rat hearts; leaves open its role and therapeutic relevance in human disease.
Biglycan mRNA expression in rat myocardium after abdominal aortic banding with renal ischemia was examined. The Northern blot analysis demonstrated that expression of biglycan mRNA in the pressure-overloaded hearts on days 2, 7, 14 and 28 was 2.88 +/- 0.89, 2.32 +/- 0.49, 2.17 +/- 0.57 and 1.81 +/- 0.46-fold higher, respectively, than that in the sham-operated hearts. In situ hybridization showed an increased density of biglycan mRNA signal-positive cells in the pressure-overloaded hearts. The cells with positive signals were spindle-shaped mesenchymal cells in the myocardial interstitium. A marked increase in biglycan mRNA signal expression was also observed in endothelial cells and smooth muscle cells of the thickened myocardial capillary wall. These results demonstrated an increase in biglycan mRNA in the pressure-overloaded heart in mesenchymal cells in the myocardial interstitium, and in endothelial and smooth muscle cells of the capillaries, indicating that biglycan contributes to the ventricular and vascular remodeling in response to pressure overload.
No takes yet. Share an insight, caveat, or question.
Ayada et al. (2001) studied Pressure-overloaded heart. Abdominal aortic banding with renal ischemia (pressure overload) vs. Sham operation was evaluated on Biglycan mRNA expression. Pressure overload induced by abdominal aortic banding in rats increased biglycan mRNA expression up to 2.88-fold in myocardial interstitial and capillary cells compared to sham operation.
Synapse has enriched 10 related papers. Consider them for comparative context: