Randomized trial characterizes epithelial responses in children with PIBO, suggesting unique immune profiles.
Key Points
This research aims to investigate the characteristics of respiratory epithelium in children with post-infectious bronchiolitis obliterans using an ex vivo model.
Analyzed proximal bronchial biopsies from children with PIBO and controls (n=3 for PIBO, n=17 for controls) using air–liquid interface culture.
Evaluated epithelial cell composition, barrier integrity, and mediator production including mucins and inflammatory responses.
Assessed differences in cytokine levels such as IL-33, TSLP, and various interferons.
Epithelial thickness was analyzed in PIBO biopsies showing no significant structural differences from controls.
Mucin expression and secretion were found to be similar in both PIBO and control groups.
PIBO epithelial cells exhibited lower IL-33 levels but higher secretion of interferon types, suggesting a heightened antiviral response.