October 10, 1988FEBS Letters
Endothelin stimulates c‐fos and c‐myc expression and proliferation of vascular smooth muscle cells
Why the study?
Does endothelin stimulate proliferation and gene expression in rat vascular smooth muscle cells?
Population
Rat vascular smooth muscle cells (VSMCs)
Design
Preclinical
Authors
Discussion
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Implication
Supports mitogenic effects of endothelin on rat VSMCs; hypothesis-generating for atherosclerosis but requires human studies.
Key Points
- This research aims to explore the effects of endothelin on the expression of c-fos and c-myc and the proliferation of vascular smooth muscle cells.
- Examined the effects of endothelin on rat vascular smooth muscle cells (VSMCs).
- Measured the elevation of intracellular calcium and its dependence on extracellular calcium.
- Assessed c-fos and c-myc mRNA levels and DNA synthesis in a dose-dependent manner.
- ETD induced a significant increase in c-fos and c-myc mRNA levels in VSMCs.
- Proliferation of VSMCs increased in a dose-dependent manner with endothelin exposure.
- Effects were linked to the potential development of atherosclerosis.
Structured PICO
Does endothelin stimulate proliferation and gene expression in rat vascular smooth muscle cells?
P
Population
Rat vascular smooth muscle cells (VSMCs)
I
Intervention
Endothelin (EDT)
O
Outcome
Intracellular calcium elevation, c-fos and c-myc mRNA levels, and DNA synthesis (proliferation)surrogate
Endothelin stimulates intracellular calcium elevation, gene expression (c-fos, c-myc), and proliferation in vascular smooth muscle cells, suggesting a potential role in the development of atherosclerosis.
Cite This Study
Komuro et al. (1988) studied this question.