Methodological report demonstrates a touchdown PCR strategy to minimize mispriming, indicating an effective way to optimize gene amplification.
Journal Article 'Touchdown' PCR to circumvent spurious priming during gene amplification Get access R.H. Don, R.H. Don Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar P. T. Cox, P. T. Cox * Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar B.J. Wainwright, B.J. Wainwright Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar K. Baker, K. Baker Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar J. S. Mattick J. S. Mattick Centre for Molecular Biology and Biotechnology, The University of QueenslandBrisbane Queensland 4072, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 14, 25 July 1991, Page 4008, https://doi.org/10.1093/nar/19.14.4008 Published: 25 July 1991 Article history Received: 04 April 1991 Published: 25 July 1991
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