October 11, 1985Science
Acquisition by innervated cardiac myocytes of a pertussis toxin-specific regulatory protein linked to the alpha 1-receptor
Key result
Sympathetic innervation of rat ventricular myocytes is associated with a 2.2- to 16-fold increase in a pertussis toxin substrate, linking alpha 1-adrenergic stimulation to decreased automaticity.
Population
Neonatal rat ventricular myocytes cultured alone or with functional sympathetic neurons, and adult rat…
Comparison
Alpha 1-agonist phenylephrine and pertussis toxin vs Myocytes cultured alone or controls without…
Design
Preclinical
Authors
Discussion
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Implication
Supports innervation-dependent alpha1 chronotropy shift in rat ventricle; leaves open translation to human electrophysiology or HF.
Structured PICO
P
Population
Neonatal rat ventricular myocytes cultured alone or with functional sympathetic neurons, and adult rat myocardium
I
Intervention
Alpha 1-agonist phenylephrine and pertussis toxin
C
Comparator
Myocytes cultured alone (uninnervated) or controls without pertussis toxin
O
Outcome
Rate of contraction and pertussis toxin-associated ADP-ribosylation of a 41-kilodalton proteinsurrogate
Main Result
Effect estimate: 2.2- and 16-fold increases
Sympathetic innervation in developing rat myocardium induces a functional pertussis toxin substrate that links alpha 1-adrenergic stimulation to a decrease in automaticity.
Cite This Study
Steinberg et al. (1985) studied Developmental changes in chronotropic response to alpha 1-adrenergic stimulation. Sympathetic innervation and pertussis toxin vs. Myocytes cultured alone (controls) was evaluated on Pertussis toxin-associated ADP-ribosylation of a 41-kilodalton protein (2.2- and 16-fold increases). Sympathetic innervation of rat ventricular myocytes is associated with a 2.2- to 16-fold increase in a pertussis toxin substrate, linking alpha 1-adrenergic stimulation to decreased automaticity.