Myrcene enhances the cardioprotective effect through matrix remodelling in an experimental model of heart failure

Figures

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  Figure 1. A–F represents the HW/BW ratio, levels of CK, LDH, and echo cardiac parameters in the control and experimental groups of rats. CK activity is expressed as μmol of phosphorus liberated/mg of protein/h. LDH activity is expressed as μmol of pyruvate liberated/mg of protein/h

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  Figure 2. A–C represents the histopathological analysis and Masson’s trichrome staining in the control and experimental group of rats (1 – Control; 2 – ISO; 3 – ISO + myrcene; 4 – Myrcene). The amount of collagen deposition was compared between groups and expressed in percentage

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  Figure 3. A–F represents the cytokines levels in the control and experimental group of rats. The detail of the experiment is given in the methodology section

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  Figure 4. A–H represents qRT-PCR mRNA expression analysis of marker genes in the control and experimental groups of rats. The fold increase of gene expression is compared with the housekeeping gene GAPDH. The detail of the experiment is given in the methodology section

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  Figure 5. A–E represents expression analysis of microRNA such as miR-21, miR-208a, miR-29, miR-19b, and miR-133 in the control and experimental groups of rats. The detail of the experiment is given in the methodology section

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  Figure 6. A–D represents the immunohistochemical (IHC) analysis of iNOS and TGF-β in the control and experimental groups of rats (1 – Control; 2 – ISO; 3 – ISO + myrcene; 4 – Myrcene). The detail of the experiment is given in the methodology section