Key result
Two-photon molecular excitation imaging successfully monitored Ca2+ transients in intact mouse hearts, revealing slower decay kinetics with fura-2 compared to rhod-2 (P<0.05).
Population
Langendorff-perfused mouse hearts
Comparison
Two-photon molecular excitation imaging with… vs Comparison between rhod-2 and fura-2 indicators…
Design
Preclinical
Authors
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Enables subcellular Ca2+ imaging in intact hearts; leaves open translation beyond mouse models.
p-value: p=<0.05
Two-photon excitation microscopy enables subcellular imaging of calcium transients in intact mouse hearts, demonstrating that fura-2 increases cytosolic Ca2+ buffering compared to rhod-2.
Rubart et al. (2003) studied Langendorff-perfused mouse hearts (n=19). Two-photon molecular excitation imaging was evaluated on Ca2+ transient decay times (p=<0.05). Two-photon molecular excitation imaging successfully monitored Ca2+ transients in intact mouse hearts, revealing slower decay kinetics with fura-2 compared to rhod-2 (P<0.05).
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