February 1, 1998AJP Heart and Circulatory Physiology
Fluorescence measurement of calcium transients in perfused rabbit heart using rhod 2
Population
Perfused rabbit hearts
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Provides reference calcium transients in intact rabbit hearts; extends rhod-2 fluorescence methods but leaves translation to human disease open.
Structured PICO
P
Population
Perfused rabbit hearts
I
Intervention
Fluorescence measurement using rhod 2 dye with reflective absorbance of excitation light as an index of dye concentration
O
Outcome
Cytosolic calcium concentration estimationsurrogate
The development of a method using rhod 2 fluorescence and reflective absorbance allows for the in vivo quantitation of cytosolic calcium transients in whole perfused rabbit hearts.
Cite This Study
Nido et al. (1998) studied this question.