November 1, 1980Endocrinology
Dependence of Aldosterone Stimulation in Adrenal Glomerulosa Cells on Calcium Uptake: Effects of Lanthanum and Verapamil
Population
Isolated adrenal glomerulosa cells
Comparison
Calcium uptake inhibitors vs Control (no calcium uptake inhibitors)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports calcium-dependent aldosterone regulation in animal adrenal cells; hypothesis-generating with no clinical implications yet.
Key Points
- To determine whether aldosterone production stimulated by angiotensin II, potassium, and ACTH in adrenal glomerulosa cells depends directly on extracellular calcium uptake.
- Incubated isolated adrenal glomerulosa cells with steroidogenic regulators (angiotensin II, potassium, and ACTH) in the presence of calcium uptake inhibitors (lanthanum and verapamil).
- Measured aldosterone, cAMP, and pregnenolone production across varying antagonist and extracellular calcium concentrations, and assessed angiotensin II receptor binding.
- Lanthanum and verapamil produced dose-dependent decreases in aldosterone production stimulated by angiotensin II, potassium, and ACTH, with only modest reductions in basal steroidogenesis.
- Verapamil raised the concentration of ACTH needed for half-maximal stimulation and inhibited angiotensin II-stimulated pregnenolone synthesis, an effect completely reversed by increasing extracellular calcium.
- Neither calcium antagonist altered the binding of angiotensin II to glomerulosa cells, demonstrating that the calcium requirement occurs at a postreceptor site.
Structured PICO
P
Population
Isolated adrenal glomerulosa cells
I
Intervention
Calcium uptake inhibitors (lanthanum and verapamil)
C
Comparator
Control (no calcium uptake inhibitors)
O
Outcome
Aldosterone productionsurrogate
Calcium uptake is essential for the stimulation of aldosterone production by angiotensin II and potassium in adrenal glomerulosa cells.
Cite This Study
Fakunding et al. (1980) studied this question.