Key result
Pretreatment with 1 microM Angiotensin II for 24 hours was required to achieve maximal desensitization (83% reduction) of AT1-mediated phospholipase C activity in bovine glomerulosa cells.
Why the study?
Does pretreatment with Angiotensin II induce desensitization of AT1 receptor-mediated cellular responses in bovine adrenal glomerulosa cells?
Population
Primary culture of bovine adrenal glomerulosa cells
Comparison
Pretreatment with Angiotensin II at various… vs Untreated cells or cells treated with lower…
Design
Preclinical
Follow-up
Up to 24 hours
Authors
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Highlights functional resistance to short-term AT1 desensitization in glomerulosa cells; leaves open translation to human physiology.
Does pretreatment with Angiotensin II induce desensitization of AT1 receptor-mediated cellular responses in bovine adrenal glomerulosa cells?
Glomerulosa cells are functionally resistant to short-term desensitization of the AT1 receptor, requiring long-term down-regulation with high concentrations of Ang II to desensitize AT1-mediated cellular responses.
Richard et al. (1997) studied this question. Angiotensin II was evaluated on Desensitization of AT1 receptor binding and phospholipase C activity. Pretreatment with 1 microM Angiotensin II for 24 hours was required to achieve maximal desensitization (83% reduction) of AT1-mediated phospholipase C activity in bovine glomerulosa cells.
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